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KMID : 0613820090190040467
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Journal of Life Science
2009 Volume.19 No. 4 p.467 ~ p.471
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Detection of a Large White-Specific Duplication in D-loop Region of the Porcine MtDNA
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Kim Jae-Hwan
Lee Jung-Gyu
Lee Sung-Soo
Cho In-Cheol
Ko Moon-Suck
Han Sang-Hyun
Jeon Jin-Tae
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Abstract
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The entire D-loop region of the porcine mitochondrial DNA (mtDNA) was amplified from six pig breeds (Landrace, Duroc, Large White, Korean native pig, Berkshire, and Hampshire) using a primer set designed on the basis of reported porcine mtDNA sequences. From analyses through cloning, DNA sequencing and multiple sequence alignment, an 11-bp (TAAAACACTTA) duplication was observed after known tandem repetition in the D-loop region, which promoted hetroplasmy in mtDNA. Although the existence of the 11-bp duplication has been previously reported in Duroc and Japanese native pigs, there have not been any attempts to know the characteristics of this duplication in other breeds so far. A 150 bp fragment containing the 11-duplication was amplified and typed by polyacrylamide gel electrophoresis (PAGE). All Large Whites had two duplication units and Duroc showed heteromorphic patterns, 11.2% (9/80) of the animals had the 11-bp duplication in total. On the other hand, Landrace, Berkshire, Hampshire and Korean native pigs were non-duplicated. This result showed that the 11-bp duplication could be used as a breed-specific DNA marker for distinguishing pure Landrace and Large White breeds.
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KEYWORD
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mtDNA D-loop, Large White, 11-bp duplication, breed-specific DNA marker
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